Effect of Lithum on adult white matter

Analysis of adult murine optic nerves cultured for 3 days in vitro (DIV) ex-vivo in organotypic media containing saline or Lithium Chloride. Results provide insight on the mechanism of action of Lithium on glial cells. Optic nerves from 6 C57BL/6 between P35-45 per group were cultured in organotypic media containing saline or Lithium Chloride (20mM) for 3DIV. Total RNA was extracted and subsequent subjected to microarray analysis using Affymetrix Mouse GeneChip 430 2.0 according to manufacturer conditions

本数据集针对经体外培养3天（体外培养天数，days in vitro，缩写为DIV）的离体成年小鼠视神经展开分析，实验采用含生理盐水（saline）或氯化锂（Lithium Chloride）的器官型培养基（organotypic media）进行培养。本研究结果可为解析氯化锂对神经胶质细胞（glial cells）的作用机制提供重要参考。每组选取6只出生后35~45日龄的C57BL/6品系小鼠，提取其视神经后置于含生理盐水或20mM氯化锂的器官型培养基中，培养3DIV。提取总RNA（Total RNA）后，依照厂商说明书的操作规范，使用Affymetrix Mouse GeneChip 430 2.0芯片开展微阵列（microarray）分析。