Expression data from polarized macrophages: effect of p53. Mus musculus

p53 is critically important in preventing oncogenesis but its role in non-cancer biology remains unclear. Macrophages exist as two subtypes (M1 and M2). Nutlin-3a (p53 activator) inhibits M2 gene expression and phenotype. p53 acts by suppressing transcription of c-Myc and thence regulates expression of a subset of M2 markers. This work has implications for our understanding of the mechanisms that regulate plasticity of macrophages in health and disease. We used microarrays to study the global programme of gene expression in nutlin-3a and 10058F4 (C-myc inhibitor) treated polarised mouse macrophages Overall design: 5 groups of cultured mouse macrophages: (i) M0 (untreated), (ii) M1, (iii) M2, (iv) M2+nutlin-3a, (v) M2+MYC inhibitor (10058F4). 3 biological replicates per treatment group.

p53在肿瘤发生的预防中发挥关键作用，但其在非肿瘤生物学中的功能仍未明确。巨噬细胞存在两种亚型（M1型与M2型）。Nutlin-3a（p53激活剂）可抑制M2型巨噬细胞的基因表达与细胞表型。p53通过抑制c-Myc的转录，进而调控部分M2型巨噬细胞标志物的表达。本研究成果有助于理解健康与疾病状态下巨噬细胞可塑性的调控机制。我们采用微阵列芯片（microarray）技术，分析经nutlin-3a与10058F4（c-Myc抑制剂）处理的极化小鼠巨噬细胞的全局基因表达谱。实验整体设计：共设置5组培养的小鼠巨噬细胞：(i) M0组（未处理），(ii) M1组，(iii) M2组，(iv) M2+nutlin-3a组，(v) M2+c-Myc抑制剂（10058F4）组。每个处理组设置3次生物学重复。