Interaction between the light environment and the Arabidopsis wound response

We have previously identified a significant increase in chloroplast reactive oxygen species in wounded leaves of Arabidopsis and other plants, which is light-dependent (Flor-Henry et al. (2004) BMC Plant Biology 4:19). The aims of this study were to (i) examine the early response to mechanical wounding in Arabidopsis leaves, (ii) test the hypothesis that light-dependent chloroplast ROS may play a role in signalling for changes in gene expression in wounded leaves, and (iii) examine the broader impact of the light environment on the wound response in Arabidopsis. Keywords: Stress response The experiment was a two-by-two factorial design, with wounding and light as the two variables. In each replicate, there are 4 treatments: unwounded/light, unwounded/dark, wounded/light, wounded/dark. Three independent biological replicate experiments were performed, with one array hybridisation performed per treatment.我们此前已在拟南芥（Arabidopsis）及其他植物的损伤叶片中检测到叶绿体活性氧（chloroplast reactive oxygen species）水平显著升高，且该变化具有光依赖性（Flor-Henry 等，2004，《BMC Plant Biology》4:19）。 本研究的研究目标包括：(i) 探究拟南芥叶片对机械损伤的早期响应；(ii) 验证“光依赖性叶绿体活性氧（ROS，Reactive Oxygen Species）可能参与损伤叶片基因表达变化的信号传导过程”这一假说；(iii) 分析光照环境对拟南芥损伤响应的更为广泛的影响。 关键词：胁迫响应（Stress response） 本实验采用二因素析因设计，以损伤处理与光照条件为两个实验变量。每个生物学重复设置4组处理：未损伤/光照、未损伤/黑暗、损伤/光照、损伤/黑暗。本研究共开展3次独立的生物学重复实验，每组处理均进行1次芯片杂交（array hybridisation）。