Nicotine effect on CEM model T cell line (kuo-affy-human-232861)

The long-term objective of this project is to establish roles played in development and function of the immune system by nicotinic acetylcholine receptors (nAChR) and exposure to the tobacco alkaloid, nicotine. The planned gene chip studies will allow us to assess effects of nicotine exposure on gene expression in a model T cell line, and the findings are expected to help direct further efforts. One of the biomedically-relevant hypotheses of this project is that tobacco use and nicotine exposure affect immune system development. To establish effects of nicotine exposure on gene expression in the CEM model T cell line. Immune system nAChR acting as ion channels or via novel signaling cascades mediate their effects on T cell development by altering expression of genes involved in T cell receptor rearrangements, cytokine expression, and cell death/survival decisions. CEM cells will be treated for one hour or one day with an effective dose of nicotine or with the same medium change but in the absence of nicotine. Keywords: nicotine, T cell line

本项目的长期目标是明确烟碱型乙酰胆碱受体（nicotinic acetylcholine receptors, nAChR）以及烟草生物碱尼古丁暴露在免疫系统发育与功能中所发挥的作用。本研究拟通过基因芯片实验，评估尼古丁暴露对模型T细胞系中基因表达的影响，所得结果可为后续研究提供方向指引。本项目提出的一项具有生物医学相关价值的假说为：烟草使用与尼古丁暴露会影响免疫系统发育。本研究旨在明确尼古丁暴露对CEM模型T细胞系中基因表达的影响。免疫系统中的烟碱型乙酰胆碱受体可作为离子通道，或通过新型信号级联反应，通过调控T细胞受体重排、细胞因子表达以及细胞死亡/存活相关基因的表达，进而介导其对T细胞发育的调控作用。将使用有效剂量的尼古丁对CEM细胞分别处理1小时与1天，同时设置仅更换同体积培养基且不含尼古丁的对照组。关键词：尼古丁，T细胞系