Gene expression in WT and podoplanin (PDPN)-deficient MEFs

MEFs were grown from WT and PDPN-deficient mouse embryos and gene expression was analyzed. The aim was to determine whether a lack of PDPN caused significant global changes in gene expression in primary fibroblasts. Here, we performed a microarray on WT and PDPN-deficient mouse embryonic fibroblasts to determine whether there were signficiant underlying changes in gene expression. Two replicates each of WT and PDPN KO MEFs from C57BL6/J mice

本研究从野生型（Wild Type，WT）及PDPN缺陷型小鼠胚胎中分离培养小鼠胚胎成纤维细胞（Mouse Embryonic Fibroblasts，MEFs），并对其基因表达水平进行分析，旨在探究PDPN缺失是否会对原代成纤维细胞的基因表达产生全局性显著改变。本研究通过基因芯片（microarray）技术对野生型与PDPN缺陷型小鼠胚胎成纤维细胞开展检测，以明确是否存在潜在的显著基因表达变化。实验所用样本为来自C57BL/6J小鼠的野生型及PDPN敲除（Knockout，KO）小鼠胚胎成纤维细胞，每组均设置两个生物学重复。