Comparative transcriptome of IgM+ cell subsets

In this study, we used RNA-Seq for comparative transcriptome profile analysis of the heterogeneity in IgM+ cell subsets from PBL of Nile tilapia, including IgM-, IgMlo and IgMhi lymphocytes. The information of transcriptome sequences and differentially-expressed genes related to innate immunity may contribute to further understanding of B cell activity and the characterization of different B cell subsets in teleost fish.

本研究采用RNA测序（RNA-Seq）对尼罗罗非鱼外周血淋巴细胞（PBL）来源的IgM阳性细胞亚群异质性开展比较转录组谱分析，所涉细胞亚群包括IgM阴性、IgM低表达及IgM高表达淋巴细胞。本研究获取的转录组序列信息及与固有免疫相关的差异表达基因数据，可为进一步理解硬骨鱼B细胞的活性及不同B细胞亚群的特征提供支撑。