Epistasis analysis of elt-1(ku491); daf-12(rh61rh411) mutant phenotypes with RNAi of other heterochronic genes.

aL4 Bursting Vulva, Alae Formation, and Young Adult Seam cell phenotypes were analyzed for strains of indicated genotypes and RNAi treatment. elt-1(rf) is the partial loss-of-function allele ku491. daf-12(lf) is the loss-of-function allele rh61rh411.bFor bursting vulva rate, the p-value for the comparison of elt-1(ku491); daf-12(lf) animals on empty-vector (-) RNAi vs target gene RNAi is lin-28, hbl-1, lin-14, lin-41 and mab-10, 0.003 for lin-46, 0.0002 for lin-42, 0.983 for kin-20, 0.0003 for ceh-16, and 0.315 for dre-1.cFor seam cell numbers, the p-value for the comparison of elt-1(ku491);daf-12(lf) animals on empty-vector (-) RNAi vs on target gene RNAi is lin-28, hbl-1, lin-14, lin-41, lin-46, lin-42, kin-20, mab-10, ceh-16, and dre-1, and > 0.9999 for lin-29.Abbreviations: e.v., empty vector; Abs, absent; Gap, gapped; Pres, present; #, seam cell number measured using the scm::GFP reporter; Std Dev, standard deviation.Epistasis analysis of elt-1(ku491); daf-12(rh61rh411) mutant phenotypes with RNAi of other heterochronic genes.

本研究针对指定基因型的线虫品系及RNA干扰（RNAi）处理样本，分析了其aL4期外阴破裂、翼状结构形成以及年轻成虫期缝合细胞（seam cell）的表型。elt-1(rf)为部分功能缺失型等位基因ku491；daf-12(lf)为功能缺失型等位基因rh61rh411。b. 针对外阴破裂率指标，对比空载（-）RNAi处理与靶基因RNAi处理的elt-1(ku491); daf-12(lf)线虫，其组间比较P值为：lin-28、hbl-1、lin-14、lin-41及mab-10，lin-46为0.003，lin-42为0.0002，kin-20为0.983，ceh-16为0.0003，dre-1为0.315。c. 针对缝合细胞计数指标，对比空载（-）RNAi处理与靶基因RNAi处理的elt-1(ku491); daf-12(lf)线虫，其组间比较P值为：lin-28、hbl-1、lin-14、lin-41、lin-46、lin-42、kin-20、mab-10、ceh-16及dre-1，lin-29的P值大于0.9999。缩写说明：e.v.代表空载载体（empty vector）；Abs代表缺失（absent）；Gap代表存在缺口（gapped）；Pres代表存在（present）；#代表通过scm::GFP报告基因检测得到的缝合细胞数量；Std Dev代表标准差（standard deviation）。本研究开展了其他异时性基因RNAi处理与elt-1(ku491); daf-12(rh61rh411)突变体表型的上位性分析。