Maternal and neonatal microbiome diversity and metabolic pathways are modified by a gastrointestinal nematode in pregnant and lactating mice

Pregnant mice were infected with Heligmosomoides bakeri nematode on gestation day 7, 12 and 17 and on postnatal day 3. Vaginal lavage was collected from dam on post-natal day 2. Pup stomach content was collected on postnatal day 2 and 7. Dam intestinal content was collected on post-natal day 2 and day 9 and dam milk was collected on postnatal day 9. After DNA isolation the samples were subjected to Miseq sequencing of bacterial 16S rRNA. Data was analysed using QIIMME2

本研究中，妊娠小鼠于妊娠第7、12、17天以及产后第3天感染巴氏鼠圆线虫（Heligmosomoides bakeri）。于产后第2天采集母鼠的阴道灌洗液；分别于产后第2天和第7天采集幼崽的胃内容物；于产后第2天、第9天采集母鼠肠道内容物，并于产后第9天采集母鼠乳汁。样本经脱氧核糖核酸（DNA）提取后，进行细菌16S核糖体RNA（16S rRNA）的Miseq测序，数据分析采用QIIME2完成。