Distinct Epithelial-Innate Immune Cell Transcriptional Circuits Underlie Airway Hyperresponsiveness in Asthma

Indirect airway hyperresponsiveness (AHR) is a highly specific feature of asthma but the underlying mechanisms responsible for driving indirect AHR remain incompletely understood. We aimed to identify differences in gene expression in epithelial brushings obtained from individuals with asthma who were characterized for indirect AHR in the form of exercise-induced bronchoconstriction (EIB). RNA-sequencing (RNA-seq) analysis was performed on epithelial brushings obtained from individuals with asthma with (n=11) and without EIB (n = 9). Bulk RNA-seq analysis comparing gene expression from epithelial brushing samples in individuals with asthma and EIB (n=11) and individual with asthma without EIB (n=9)

间接气道高反应性（indirect airway hyperresponsiveness, AHR）是哮喘的高度特异性特征，但驱动间接AHR发生的潜在机制仍未完全阐明。本研究旨在明确以运动诱发性支气管收缩（exercise-induced bronchoconstriction, EIB）为表型的哮喘患者上皮刷检样本中的基因表达差异。研究对伴EIB的哮喘患者（n=11）与不伴EIB的哮喘患者（n=9）的上皮刷检样本开展了批量RNA测序（bulk RNA-seq）分析，对比两组间的基因表达水平。