Porcine ZC3H11A is essential for the proliferation of pseudorabies virus (PRV) and porcine circovirus 2 (PCV2) by mediating mRNA export

Purpose:ZC3H11A has been reported to play a role in RNA transport. The purpose of this study was to detect the differentially expressed genes after ZC3H11A knockout. Methods: RNA sequencing was performed on PK-15 cell line (WT) and ZC3H11A-/- using Illumina platform, and three repeated RNA sequencing results were obtained. Results: Using an optimized data analysis workflow,different expression genes were obtained and RNA transport pathway was enriched through KEGG pathway analysis. Conclusions: In this study, we observed that ZC3H11A interacted with TRanscription and the EXport complex (TREX) and played an important role in mRNA export. Overall design: PK-15 cell line (WT) and ZC3H11A-/- cells

研究目的：已有研究证实ZC3H11A参与RNA转运过程。本研究旨在检测ZC3H11A基因敲除后的差异表达基因。
研究方法：本研究依托Illumina测序平台，对PK-15细胞系（野生型，WT）及ZC3H11A基因敲除型（ZC3H11A-/-）开展RNA测序，共获得3组生物学重复的RNA测序数据。
研究结果：通过优化后的数据分析流程，本研究筛选得到差异表达基因，并经KEGG通路富集分析发现RNA转运通路显著富集。
研究结论：本研究发现ZC3H11A可与转录-输出复合物（Transcription and the EXport complex, TREX）相互作用，并在mRNA输出过程中发挥重要作用。
实验设计：包含PK-15野生型细胞系（WT）及ZC3H11A基因敲除细胞。