Primary human Hepatic Stellate Cells and PNPLA3 I148M

A) RNA sequencing was performed on 2D-cultured primary human Hepatic Stellate Cells (HSC) genotyped for the PNPLA3 I148M variant. 

B) RNA sequencing from liver biopsies of genotyped PNPLA3 patients, previously published (http:// dx. doi. org/ 10. 1136/gutjnl- 2019- 319226).

C) Comparison of both datasets of A and B.

D) Cell behaviour was evaluated in HSCs wild type (WT) or carrying I148M PNPLA3 cultured on 3D extracellular matrix (ECM) scaffolds from human healthy and cirrhotic liver with/without TGFB1 and NanoString was employed.  

All datasets are available in Excel files.

A) 对经PNPLA3 I148M变异基因分型的2D培养原代人肝星状细胞（Hepatic Stellate Cells, HSC）开展RNA测序。
B) 数据来源于经基因分型的PNPLA3患者肝活检样本的RNA测序，该数据集此前已发表（链接：http://dx.doi.org/10.1136/gutjnl-2019-319226）。
C) 对数据集A与数据集B进行对比分析。
D) 针对在人类健康及肝硬化来源的3D细胞外基质（Extracellular Matrix, ECM）支架上培养的野生型（Wild Type, WT）或携带I148M PNPLA3变异的肝星状细胞，评估其细胞行为；实验设置了添加与不添加转化生长因子β1（TGFB1）的组别，并采用了NanoString技术进行检测。
所有数据集均以Excel文件格式提供。