Penium margaritaceum Transcriptome. Penium margaritaceum

Green algal cultures were grown up in appropriate medium at 18°C and a 12:12 LD photoperiod with a photon flux of 180-200 %u03BCmol s-1 m-2. RNA was isolated during log phase growth in a variety of conditions to maximize the diversity of transcripts: at intervals at 7 am, 12 pm, 4 pm and 9 pm; after sitting in a dark enclosure for 24 hours; and after being exposed to 20 min of -20°C. This evolutionary study was performed to collect genomic scale data on the charophyte lineages most closely related to land plants. Research questions derived from these data include phylogenomic analyses and uncovering the origin of plant molecular processes.

绿藻培养物于适宜培养基中，在18℃、12:12光暗（LD）光周期及180-200 μmol·s⁻¹·m⁻²光子通量密度的条件下培养。本研究在对数生长期的多种条件下分离RNA以最大化转录本多样性：分别于上午7时、中午12时、下午4时与晚上9时取样；将藻体置于黑暗密闭环境中培养24小时后取样；以及将藻体暴露于-20℃环境20分钟后取样。本进化研究旨在收集与陆生植物亲缘关系最为相近的轮藻（charophyte）谱系的基因组规模数据，基于上述数据衍生的研究问题包括系统发育基因组学分析以及植物分子过程起源的揭示。