Expression data from human primary cumulus cells culture (hCC). Homo sapiens

The cumulus cells niche that surrounds the oocyte is essential for its maturation and presumably for the oocyte to acquire its competence to confer pluripotency. The cells cultured from the human oocyte cumulus niche (hCC) could be used as feeders for the propagation of human pluripotent stem cells in vitro. We used microarrays to establish the molecular portrait of the hCC at different passages Overall design: Cumulus cells were dissociated mechanically from the oocyte and cultured for a long period. Affymetrix Human Genome U133 Plus 2.0 DNA chips were performed to analyze the transcriptome of hCC at different passages (P3, P6 and P9). The Affymetrix 3’ IVT express protocol was used to prepare cRNA (one-cycle amplification). The gene expression data were extracted using Affymetrix Expression Console software.

围绕卵母细胞（oocyte）的卵丘细胞微环境，对其成熟过程至关重要，且可帮助卵母细胞获得赋予多能性的能力。从人卵母细胞卵丘微环境中分离培养的细胞（hCC），可作为饲养层细胞，用于体外扩增人多能干细胞。本研究通过基因芯片技术，构建了不同传代阶段hCC的分子表达谱。

实验设计：通过机械解离法从卵母细胞中分离卵丘细胞并进行长期体外培养；采用Affymetrix Human Genome U133 Plus 2.0基因芯片，分析不同传代阶段（P3、P6、P9）hCC的转录组；使用Affymetrix 3’ IVT express试剂盒流程制备cRNA（单轮扩增法）；通过Affymetrix Expression Console软件提取基因表达数据。