AFLP primer combinations and fluorescent dyes.

The first column contains the different MseI-based primers used. The next four columns contain the fluorescently labeled EcoRI-based primers that were used in combination with the MseI-based primer within the same row. The primers are 19 bp in length and consist of a 16 bp core sequence and a 3 bp extension. ��m�� is short for a GATGAGTCCTGAGTAA core sequence and ��e�� stands for a GACTGCGTACCAATTC core sequence. ��m�� and ��e�� are followed by the three base extensions that differentiate them. The colors of the fluorescent labels of the EcoRI-based primers are presented in the column headers, and the fluorescent 5�� modifications in the cells below them (5-FAM, 6-FAM, JOE, VIC, NED and PET). Individual AFLP markers in Fig. 2 & 3 are characterized by the eNNN-mNNN combinations shown in this table and the PCR product size. The final column describes which fragment analysis instrument was used.

第一列包含本实验所用的各类基于MseI的引物。后续四列包含与同一行内MseI基引物配对使用的、带有荧光标记的基于EcoRI的引物。此类引物全长19个碱基对（bp），由16 bp的核心序列与3 bp的延伸序列组成。[m]为GATGAGTCCTGAGTAA核心序列的简写，[e]则代表GACTGCGTACCAATTC核心序列的简写。[m]与[e]后接用于区分二者的3个碱基延伸序列。基于EcoRI的引物的荧光标记颜色标注于列标题中，其下方单元格内则标注了引物的5'-末端荧光修饰类型（5-FAM、6-FAM、JOE、VIC、NED及PET）。图2与图3中的单个AFLP标记（Amplified Fragment Length Polymorphism，扩增片段长度多态性）可通过本表所示的eNNN-mNNN组合与PCR产物片段长度进行表征。最后一列说明了本次实验所使用的片段分析仪器。