Gene expression data in estrogen receptor alpha positive breast tumors with and without PIK3CA mutations.. Homo sapiens

PI3K/AKT pathway plays one of pivotal roles in breast cancer development and maintenance. PIK3CA, coding PIK3 catalytic subunit, is the oncogene which shows the high frequency of gain-of-function mutations leading to the PI3K/AKT pathway activation in breast cancer. In particular in the ERα-positive breast tumors PIK3CA mutations have been observed in 30% to 40%. However, genes expressed in connection to the pathway activation in breast tumorigenesis remain largely unknown. To identify downstream relevant target genes (and signaling pathways) turned on by the aberrant PI3K/AKT signal in breast tumors, we analyzed gene expression by pangenomic oligonucleotide microarray in a series of 43 ERα-positive tumors with and without PIK3CA mutations. Overall design: 43 ERα-positive breast tumors including 14 tumors with PIK3CA mutations and 29 tumors without PIK3CA mutattions were used as screening set for microarray.

磷脂酰肌醇3-激酶/蛋白激酶B（PI3K/AKT）信号通路在乳腺癌的发生与维持中发挥关键作用。编码PI3K催化亚基的PIK3CA是一种致癌基因，在乳腺癌中高频出现功能获得性突变，进而激活PI3K/AKT信号通路。其中，在雌激素受体α（ERα）阳性乳腺肿瘤中，PIK3CA突变发生率可达30%~40%。然而，目前对于乳腺癌发生过程中与该通路激活相关的表达基因仍知之甚少。为了鉴定乳腺癌中由异常PI3K/AKT信号激活的下游相关靶基因（及信号通路），本研究采用全基因组寡核苷酸微阵列（pangenomic oligonucleotide microarray）技术，对43例携带与不携带PIK3CA突变的ERα阳性乳腺肿瘤进行了基因表达分析。实验整体设计：本研究共纳入43例ERα阳性乳腺肿瘤，其中14例携带PIK3CA突变、29例不携带该突变，将其作为微阵列筛选的样本集。