RNA sequencing of human airway epithelial cultures from cystic fibrosis (CF) and non-CF donors exposed to tumor necrosis factor-alpha and interleukin-17 (TNFα+IL-17) for 5 hours. RNA sequencing of human airway epithelial cultures from cystic fibrosis (CF) and non-CF donors exposed to tumor necrosis factor-alpha and interleukin-17 (TNFα+IL-17) for 5 hours

Gene expression was compared between disease conditions (CF vs. non-CF) and treatments (TNFα+IL-17 vs. control) as well as the interaction between disease and treatment. Overall design: Primary differentiated airway epithelia were treated with TNFα (10ng/ml) and IL-17 (20ng/ml) for 5 hours, prepared for RNA sequencing, and sequenced on Illumina HiSeq 4000 system using 2x150bp paired end SBS chemistry.

本研究对疾病状态（囊性纤维化（Cystic Fibrosis, CF）与非囊性纤维化）、处理条件（肿瘤坏死因子α（Tumor Necrosis Factor α, TNFα）联合白细胞介素17（Interleukin 17, IL-17）与对照组）下的基因表达水平，以及疾病状态与处理条件的交互作用进行了比较分析。整体实验设计如下：原代分化气道上皮细胞经10ng/ml TNFα与20ng/ml IL-17联合处理5小时后，完成RNA测序文库制备，随后基于Illumina HiSeq 4000测序平台，采用2×150bp双端边合成边测序（Sequencing by Synthesis, SBS）化学方法完成测序。