Rational design of a multiepitope vaccine encoding T-lymphocyte epitopes for treatment of chronic hepatitis B virus infections.

Sequences from 20 HBV isolates, including adr, adw, ayr, and ayw serotypes, were analyzed using text string search software to identify amino acid sequences of 15 or 20 amino acids containing HLA-DR supertype motifs. Peptide sequences that were represented in at least 45% of the HBV sequences were synthesized and tested.

本研究对20株乙型肝炎病毒（HBV）分离株的序列进行分析，这些分离株包含adr、adw、ayr、ayw四种血清型；采用文本字符串搜索软件，筛选出含有人类白细胞抗原DR（HLA-DR）超型基序、长度为15或20个氨基酸的氨基酸序列；将在至少45%的HBV序列中出现的肽序列进行合成并开展实验验证。