Identification and analysis of oil candidate genes reveals the molecular basis of cottonseed oil accumulation in Gossypium hirsutum L.

<b>Table S1. </b><b> </b>ANOVA and broad-sense heritability analysis for cottonseed oil content.<b></b> <b>Table S2. </b>QTLs related to cottonseed oil content identified in eight environments. <b>Table S3. </b>832 genes in the five stable QTL regions. <b>Table S4.</b> KEGG pathway enrichment analysis of 832 genes around stable QTLs. <b>Table S5.</b> Genes list identified by BLAST with <i>Arabidopsis thaliana</i> oil-related genes. <b>Table S6. </b>The expression level (FPKM values) of candidate oil genes during developing ovules of <i>G. hirsutum</i> L.<b></b> <b>Table S7. </b>Four transcription factors related to cottonseed lipid content. <b>Table S8. </b>The microRNA-target gene regulatory network of <i>G. hirsutum</i> L. <b>Table S9.</b> The designed primers of genes encoding the key enzymes involved in lipid biosynthesis for qRT-PCR.

**补充表S1.** 棉籽油含量的方差分析（Analysis of Variance，ANOVA）与广义遗传力分析。**补充表S2.** 8个环境中鉴定到的与棉籽油含量相关的数量性状位点（Quantitative Trait Locus，QTL）。**补充表S3.** 5个稳定数量性状位点区域内的832个基因。**补充表S4.** 稳定数量性状位点侧翼832个基因的京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析。**补充表S5.** 与拟南芥（Arabidopsis thaliana）油脂相关基因通过局部序列比对基本工具（Basic Local Alignment Search Tool，BLAST）比对鉴定得到的基因列表。**补充表S6.** 陆地棉（Gossypium hirsutum L.）发育胚珠中候选油脂基因的表达水平（每百万映射读取的每千碱基转录本片段数，Fragments Per Kilobase of transcript per Million mapped reads，FPKM）。**补充表S7.** 4个与棉籽脂质含量相关的转录因子。**补充表S8.** 陆地棉（Gossypium hirsutum L.）的微小RNA（microRNA）-靶基因调控网络。**补充表S9.** 脂质生物合成关键酶编码基因的实时荧光定量聚合酶链反应（quantitative Real-Time Polymerase Chain Reaction，qRT-PCR）引物设计序列。