Whole-genome microarray analysis was performed on cells treated with 5 μM dTAT for 1h

Analysis of the impacto of dTAT treatment on primary human dermal flibroblast at the gene expression level. Results show that dTAT cytosolic penetration has a minimal impact on mRNA expression and that cells rapidly recover from treatment with dTAT. Primary Human dermal Fibroblast (HDF) were treated with dTAT (5µM) for 1h. Total RNA was harvested from HDF cells immediately, 1h or 24h after treatment.

本研究针对dTAT处理在基因表达水平上对原代人皮肤成纤维细胞（primary human dermal fibroblast，简称HDF）的影响展开分析。结果显示，dTAT的胞质穿透作用对mRNA表达仅存在极微弱影响，且经dTAT处理后的细胞可快速恢复正常状态。实验中，原代人皮肤成纤维细胞以5µM浓度的dTAT处理1小时，分别于处理后即刻、1小时及24小时收集细胞总RNA。