Additional file 1 of Biological evaluation of novel gemcitabine analog in patient-derived xenograft models of pancreatic cancer

Additional file 1: Supplementary Fig 1. A (SF 1A). HPLC spectrum for detecting the purity of 4NSG at 98.8% with a wavelength of 254 nm. B (SF 1B). Nuclear magnetic resonance spectrum for 4NSG. (a) 4NSG proton nuclear magnetic resonance (1H NMR) peaks at 10.88 ppm (-CO-NH) and 1.38–1.04 ppm (-CH2)15 representing the amide linkage and long-chain methylene group contributed by stearic acid respectively. (b) amide carbonyl carbon in 4NSG displays a characteristic C-13 NMR peak at 174.55 ppm. C (SF 1C). Mass spectrum for 4NSG at 530.59 m/z.

附加文件1：补充图1。A（SF 1A）：采用254 nm波长检测4NSG纯度的高效液相色谱（High Performance Liquid Chromatography, HPLC）谱图，4NSG纯度达98.8%。B（SF 1B）：4NSG的核磁共振谱图。(a) 4NSG的质子核磁共振（Proton Nuclear Magnetic Resonance, 1H NMR）谱峰中，10.88 ppm处为(-CO-NH-)酰胺键的特征峰，1.38~1.04 ppm处的(-CH2)15峰则对应硬脂酸所贡献的长链亚甲基基团。(b) 4NSG中的酰胺羰基碳在174.55 ppm处呈现特征碳13核磁共振（Carbon-13 Nuclear Magnetic Resonance, 13C NMR）峰。C（SF 1C）：4NSG的质谱图，其特征质荷比（mass-to-charge ratio, m/z）为530.59。