Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors

We examined genome-wide DNA methylation with 20 human iPSC lines derived from five different cell types,and 7 human ESC lines using Illumina’s Infinium HumanMethylation450K and compared among iPSCs by retrovirus, Sendai virus or episomal vectors. Bisulphite converted DNA from the 30 samples were hybridised to the Illumina Infinium Human Methylation450K Beadchip

本研究采用Illumina Infinium HumanMethylation450K芯片，对20株源自5种不同细胞类型的人诱导多能干细胞（induced pluripotent stem cell, iPSC）及7株人胚胎干细胞（embryonic stem cell, ESC）开展全基因组DNA甲基化检测；其中人诱导多能干细胞分别通过逆转录病毒、仙台病毒或附加型载体介导重编程构建。研究对30份样本的亚硫酸氢盐转化DNA进行Illumina Infinium HumanMethylation450K Beadchip芯片杂交，并针对不同重编程方法获得的人诱导多能干细胞开展组间比较分析。