Differential effects of maternal high protein diets on liver transcriptomes

The objective of this study was to test if maternal high protein diet during pregnancy or lactation, imposes different transient or persistently imprinted effects on the liver transcriptome of offspring Livers from 4 to 5 animals (one animal per litter) at days 21 and 150 of of each dietary group were combined to 3 pools each, and processed for microarray analysis.

本研究旨在探究妊娠或泌乳期母体摄入高蛋白饮食，是否会对子代肝脏转录组（liver transcriptome）产生不同的瞬时或持续性印记效应（imprinted effects）。实验中，各膳食处理组选取4~5只动物（每窝仅取1只），分别在第21日龄和第150日龄采集其肝脏样本；将每个时间点的样本合并为3个混合样本池，随后开展微阵列分析（microarray analysis）。