RNAseq of FACS sorted testes from Mus X-Y mismatch experimental crosses

We tested if X-Y match from the same Mus musculus subspecies on an F1 hybrid autosomal background rescued sterility effects and misexpression seen in sterile F1 Mus hybrids. We also tested if X-Y mismatch on a non-hybrid background led to sterility and misexpression. We compared expression in X-Y rescue and X-Y mismatch cross types to control F1 mice using transcriptomic data from cell populations sorted from mouse whole testes.

我们开展实验以验证：在F1杂交常染色体背景下，来自同一小家鼠（Mus musculus）亚种的X-Y染色体匹配，是否能够挽救不育F1小家鼠杂交个体中出现的不育效应与表达紊乱现象。同时，我们还验证了非杂交背景下的X-Y染色体不匹配是否会引发不育与表达紊乱。我们利用从小鼠全睾丸中分选的细胞群体的转录组数据，将X-Y染色体挽救杂交组与X-Y染色体不匹配杂交组的基因表达水平与对照F1小鼠进行了对比分析。