Expression data from PDVC57 cells stimulated with or without Onscostatin M. Expression data from PDVC57 cells stimulated with or without Onscostatin M

Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer in caucasian population and it's important to find new therapeutic targets. Cytokines expressed in tumor microenvironment can have strong effects on tumor cell phenotype. We have characterized the effect of Oncostatin M (OSM), a cytokine belonging to the IL-6 familly on the transcriptome of transformed keratinocytes cell line, PDVC57 cells. In this dataset, we have included the expression data obtained from transformed keratinocytes PDVC57 cells stimulated or not with OSM for 6 or 24 hours. These data have shown that 32 genes were differentially expressed in response to OSM stimulation. Overall design: 4 samples were analyzed, non stimulated (NS) for 6 or 24 hours used as controls and treated with OSM (10 ng/mL) for 6 or 24 hours. We generated the following pairwise comparisons NS 6h vs OSM 6h and NS 24h vs OSM 24h. Genes with a fold-change ≤0.5 or a fold-change ≥2 were selected.

皮肤鳞状细胞癌（Cutaneous squamous cell carcinoma, cSCC）是白种人群中第二常见的皮肤癌，探寻新型治疗靶点至关重要。肿瘤微环境中表达的细胞因子可对肿瘤细胞表型产生强烈调控作用。本研究针对抑瘤素M（Oncostatin M, OSM）——一种隶属于IL-6家族的细胞因子——对转化型角质形成细胞系PDVC57的转录组（transcriptome）的调控效应进行了系统表征。本数据集收录了经OSM刺激或未刺激（non stimulated, NS）6小时、24小时的PDVC57细胞的基因表达数据。分析结果显示，共有32个基因在OSM刺激后呈现差异表达。
实验整体设计：共分析4组样本，分别为未刺激6小时、未刺激24小时的对照组，以及经10 ng/mL OSM处理6小时、处理24小时的实验组。本研究开展了两组配对比较：NS 6小时组vs OSM刺激6小时组，NS 24小时组vs OSM刺激24小时组。筛选阈值为折叠变化（fold-change）≤0.5或≥2的基因。