Web based scoring is useful for validation and harmonisation of scoring criteria within RENEB

<b>Purpose:</b> To establish a training data set of digital images and to investigate the scoring criteria and dose assessment of the dicentric assay within the European network of biodosimetry (RENEB), a web based scoring inter-comparison was undertaken by 17 RENEB partners. <b>Materials and methods:</b> Two sets of 50 high resolution images were uploaded onto the RENEB website. One set included metaphases after a moderate exposure (1.3 Gy) and the other set consisted of metaphases after a high dose exposure (3.5 Gy). The laboratories used their own calibration curves for estimating doses based on observed aberration frequencies. <b>Results:</b> The dose estimations and 95% confidence limits were compared to the actual doses and the corresponding z-values were satisfactory for the majority; only the dose estimations from two laboratories were too low or too high. The coefficients of variation were 17.6% for the moderate and 11.2% for the high dose. Metaphases with controversial results could be identified for training purposes. <b>Conclusions:</b> Overall, the web based scoring of the two galleries by the 17 laboratories produced very good results. Application of web based scoring for the dicentric assay may therefore be a relevant strategy for an operational biodosimetry assistance network.

**目的：** 为构建数字化图像训练数据集，并在欧洲生物剂量学网络（RENEB）框架内探究双着丝粒试验（dicentric assay）的评分标准与剂量评估方法，17家RENEB合作机构开展了基于网络的评分比对研究。

**材料与方法：** 将两组各50幅高分辨率图像上传至RENEB网站。一组包含受照剂量为1.3 Gy的中度暴露组细胞分裂中期相，另一组则为受照剂量3.5 Gy的高剂量暴露组细胞分裂中期相。各实验室基于观测到的染色体畸变频率，使用自有校准曲线开展剂量估算。

**结果：** 将剂量估算值与95%置信限与实际受照剂量进行比对，多数实验室的z值均符合要求；仅两家实验室的剂量估算值偏高或偏低。中度暴露组的变异系数为17.6%，高剂量暴露组为11.2%。可筛选出存在争议结果的细胞分裂中期相用于后续训练。

**结论：** 总体而言，17家实验室对两组图像集开展的基于网络的双着丝粒试验评分取得了优异结果。因此，将基于网络的评分方法应用于双着丝粒试验，或可成为运行式生物剂量学援助网络的有效策略。