RNA sequence reads of Musa acuminata Colla Plantlets in Response to Stress

Salinity and drought condition have been the major problems that limit the production yield of banana. The aim of study is to reveal the molecular mechanisms of banana plantlets in response to salinity and drought stresses using the transcriptome analysis approach. We report a transcriptome dataset generated from five cDNA libraries of banana plantlet groups which were exposed to salinity and drought stress treatments. Sodium chloride at 100 mg/l was added in culture medium to induce salinity stress and polyethylene glycol (PEG-6000) was added at 2.5%, 7.5%, and 10% to induce water deficiency condition. Plantlets grown on in vitro culture medium without stress treatment were used as the control group. Plantlets of banana, Musa acuminata Colla (AAA genome; cv Barangan Merah) were in vitro regenerated from the salinity and drought tolerant shoots and utilized as the source of total RNA samples for the Next Generation Sequencing (NGS). The five cDNA libraries were sequenced using both Illumina MiSeqTM 2000 and HiSeqTM 2000 platform resulting a transcriptome dataset with ten cDNA libraries.

盐胁迫与干旱胁迫是制约香蕉产量的主要逆境因子。本研究旨在通过转录组（transcriptome）分析方法，解析香蕉组培苗响应盐胁迫与干旱胁迫的分子调控机制。本研究公开了一套转录组数据集，该数据集来源于经盐胁迫、干旱胁迫处理的香蕉组培苗群体构建的5个cDNA文库。实验通过在培养基中添加100 mg/L的氯化钠以诱导盐胁迫，同时分别添加2.5%、7.5%、10%浓度的聚乙二醇（PEG-6000）以诱导水分缺失胁迫。以未施加任何胁迫处理的离体培养基培养的香蕉组培苗作为对照组。本研究所用的香蕉组培苗为从小果野蕉（Musa acuminata Colla，AAA基因组；品种Barangan Merah）的耐盐耐旱芽离体再生获得的材料，其总RNA样品用于下一代测序（Next Generation Sequencing, NGS）。本研究采用Illumina MiSeq™ 2000与HiSeq™ 2000测序平台对上述5个cDNA文库进行测序，最终获得包含10个cDNA文库的转录组数据集。