Chip-seq analyses of PPAR? bonded DNA fragments

Chip-seq analyses of PPAR? bonded DNA fragments revealed that Arid5b, Rorb, Pank2 and Htr2c are the downstream target genes for PPAR? regulated adipogenesis in fetal preadipocyte. Overall design: Chip-seq of 3T3-L1 preadipocytes and PPAR?-OE HTR8-Exos treated 3T3-L1 preadipocytes

针对过氧化物酶体增殖物激活受体γ（PPARγ）结合DNA片段的染色质免疫共沉淀测序（Chip-seq）分析结果显示，Arid5b、Rorb、Pank2及Htr2c是PPARγ调控胎儿前体脂肪细胞脂肪生成过程的下游靶基因。实验设计概述：本研究对3T3-L1前体脂肪细胞，以及经过表达PPARγ的HTR8细胞外囊泡处理的3T3-L1前体脂肪细胞开展了Chip-seq测序。