Role of HIP1 in HIPPI mediated transcription regulation in HeLa cells. Homo sapiens

Nuclear entry of transcription factor HIPPI is mediated by its interacting partner Huntingtin Interacting Protein 1 (HIP1), a nuclear localization signal containing nucleo-cytoplasmic shuttling protein. In oredr to investigate the role of HIP1 in HIPPI mediated transcriptional regulation in cell, here we performed microarray experiments using stable HIP1 knocked down HeLa cells (Hip1Si) exogenously expressing Green fluorescent protein tagged HIPPI. Overall design: Total RNA extracted from HIP1 knocked down HeLa cells (Hip1Si) transfected with empty GFP vector served as control and Total RNA extracted from Hip1Si cells transfected with GFP-Hippi construct served as test. Biological replicates: 4

转录因子HIPPI的核输入由其相互作用伴侣亨廷顿相互作用蛋白1（Huntingtin Interacting Protein 1，HIP1）介导，该蛋白是一类含有核定位信号的核质穿梭蛋白。为探究HIP1在HIPPI介导的细胞转录调控中的作用，本研究针对稳定敲低HIP1的HeLa细胞（命名为Hip1Si），在外源表达绿色荧光蛋白（Green fluorescent protein，GFP）标签的HIPPI的体系中开展微阵列实验。
总体实验设计：以转染空GFP载体的Hip1Si细胞提取的总RNA作为对照样本，以转染GFP-Hippi融合表达构建体的Hip1Si细胞提取的总RNA作为实验组样本。生物学重复次数为4次。