Investigation into whether the RD2 element in serotype M28 isolates of group A Streptococcus influences global gene expression

We used RNA-Seq to compare a parental M28 isolate with an isogenic mutant in which the RD2 element had been deleted. Overall design: Parental M28 isolate MGAS6180 and an RD2 deletion mutant derivative (M28?RD2) were compared following growth in THY broth to the exponential phase of growth (an O.D.600 of 0.5).

本研究采用RNA测序（RNA-Seq）技术，对亲本M28分离株与RD2元件缺失的同基因突变株进行对比分析。实验设计概述：将在THY肉汤（THY broth）中培养至对数生长期（光密度600nm值为0.5）的亲本M28分离株MGAS6180及其RD2缺失衍生突变株（M28ΔRD2）进行对比分析。