Bulk RNA-seq for undifferentiated spermatogonia from the testes of control, aged and busulfan-treated C57BL/6 mice

Samples were prepared from 100 GFRa1-GFP positive, Miwi2-tdTom negative and 100 GFRa1-GFP negative, Miwi2-tdTom positive cells that were sorted with FACS. cDNA libraries were prepared with the SMARTseq2 protocol. Adult, control samples had four biological replicates while the aged and busulfan-treated samples were in triplicate. There were 2 technical replicates per biological sample.

本数据集的样本通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）分选出100个GFRα1-绿色荧光蛋白（GFRa1-GFP）阳性且Miwi2-tdTomato（Miwi2-tdTom）阴性的细胞，以及100个GFRα1-绿色荧光蛋白（GFRa1-GFP）阴性且Miwi2-tdTomato（Miwi2-tdTom）阳性的细胞制备获得。随后采用SMARTseq2实验流程构建互补DNA（complementary DNA，cDNA）文库。其中成年对照组样本设置4个生物学重复，衰老组与白消安处理组样本各设置3个生物学重复，且每个生物学样本均设置2个技术重复。