Xdrop - Oxford Nanopore long read sequencing of HAP1 and HepG2 tRNA gene deletion clones. Xdrop - Oxford Nanopore long read sequencing of HAP1 and HepG2 tRNA gene deletion clones

This dataset contains Xdrop followed by oxford nanopore long read sequencing performed in target tRNA gene deletion clones in HAP1 (t72) and HepG2 (t15). By applying de novo assembly based approach to Xdrop-LRS data, we identified Cas9-induced on-target genomic alteration.

本数据集包含在HAP1细胞系（t72）与HepG2细胞系（t15）的靶向tRNA基因缺失克隆株中开展的Xdrop联合牛津纳米孔长读长测序（Oxford Nanopore Long Read Sequencing）实验数据。通过对Xdrop-LRS数据应用基于从头组装的分析方法，本研究成功鉴定出Cas9诱导的靶向基因组变异。