miRNA expression profiling of enriched male germ cell populations. Mus musculus

MicroRNA is essential for the process of spermatogonesis, however analysis of its change in expression within germ cells during this process has been limited. We set out to examine the change in the miRNA expression profile of highly enriched mouse germ cell populations in vairous developmental stages.These inlcuded postnatal gonocytes, day 7-9 spermatogonia as well as pachytene spermatocytes and round spermatids from adult mice Overall design: Gonocytes (day1) and spermatogonia (day 7-9), pachytene spermatocytes and round spermadits (adult) were enrichged by 2-4% BSA gradient sedementation. Three biological replicates of each cell type were included.

微小RNA（MicroRNA）在精子发生（spermatogonesis）过程中具有重要功能，然而针对该过程中生殖细胞内微小RNA表达变化的分析却相对匮乏。本研究旨在探究不同发育阶段下高度富集的小鼠生殖细胞群的miRNA表达谱变化，所涉及的细胞群包括出生后生殖母细胞、7-9日龄精原细胞，以及成年小鼠来源的粗线期精母细胞与圆形精子细胞。
实验设计概述：通过2-4%牛血清白蛋白（BSA）梯度沉降法对生殖母细胞（出生后第1天）、7-9日龄精原细胞、成年小鼠粗线期精母细胞及圆形精子细胞进行富集。每种细胞类型均设置3次生物学重复。