An extremely sensitive nested PCR-RFLP mitochondrial marker for detection and identification of salmonids in eDNA from water samples

Supplemental material and sequences obtained from the paper "An extremely sensitive nested PCR-RFLP mitochondrial marker for detection and identification of salmonids in eDNA from water samples".
- Files "peerj-13885-Supplementary_table_1 and peerj-13885-Supplementary_table_2" are the supplemental material, and include the species used for cross amplification and extra sensitivity assays for the family specific primers designed.
- In the file "Sequences_16S_tissue" are the sequences obtained with universal primers from different fishes species used in the cross-amplification tests. The Genbank accession numbers are indicated in each sequence.
- In the file "Sequences_specific_primer_tissue" are the sequences obtained with the family specific primer for the five salmonids described in the paper. The Genbank accession numbers are indicated in each sequence.
- In the file "Sequences_specific_primer_eDNA" are the sequences obtained with the family specific primer for the  environmental DNA samples of the positive controls: Nora River and El Arenero. The Genbank accession numbers are indicated in each sequence.

本数据集的补充材料与序列均源自论文《用于检测和鉴定水样中环境DNA（environmental DNA, eDNA）内鲑科鱼类的超高灵敏度嵌套聚合酶链反应-限制性片段长度多态性（nested PCR-RFLP）线粒体标记》。
- 文件"peerj-13885-Supplementary_table_1"与"peerj-13885-Supplementary_table_2"为本次补充材料，包含了针对所设计的科特异性引物开展交叉扩增实验与额外灵敏度验证实验的受试物种。
- 文件"Sequences_16S_tissue"中收录了利用通用引物从交叉扩增实验所用的不同鱼类物种中获取的序列，每条序列均标注了其基因银行（GenBank）登录号。
- 文件"Sequences_specific_primer_tissue"中收录了利用论文中所述的5种鲑科鱼类的科特异性引物获取的序列，每条序列均标注了其基因银行（GenBank）登录号。
- 文件"Sequences_specific_primer_eDNA"中收录了利用科特异性引物从阳性对照环境DNA样本（诺拉河与埃尔阿雷内罗样本）中获取的序列，每条序列均标注了其基因银行（GenBank）登录号。