Thermodynamic parameters derived from the microcalorimetric titrations of peptide BACHEMP-Cons in different buffer systems.

The values shown are means and standard deviations from three individual experiments.aPB, Polybuffer (5 mM Tris, 5 mM MES, 5 mM PIPES).bUnder the experimental conditions used (titration of 50 µM peptide with 5 mM salt solution), which corresponds to a final ligand concentration in the sample cell of 900 µM.

所示数值为三次独立实验所得的均值与标准差。aPB：多缓冲液（Polybuffer），组分包含5 mM 三(羟甲基)氨基甲烷（Tris）、5 mM 2-(N-吗啉代)乙磺酸（MES）与5 mM 哌嗪-N,N'-二(2-乙磺酸)（PIPES）。b 在所采用的实验条件下（以5 mM盐溶液滴定50 µM肽段），样品池内配体的最终浓度为900 µM。