Saccharum hybrid cultivar SP80-3280 cultivar:SP80-3280 Transcriptome or Gene expression. Saccharum hybrid cultivar SP80-3280 cultivar:SP80-3280

Sugarcane one-eyed seed sets (genotype SP80-3280, CTC, Brazil) were planted in 200 ml plastic cups containing a commercial planting mix (Plantmax, Eucatex) and cultivated for 20 days under greenhouse conditions. The plantlets were subsequently transferred to a growth chamber at 26°C on a 16 h/8 h light/dark cycle with a photon flux density of 70 µE.m-2.s-1 to acclimate. Plantlets were then sprayed with a 100 µmol.L-1 MeJA solution (Bedoukian Research Inc., Danbury, CT), whereas control plantlets were treated with distilled water. Leaves were collected 0, 1, 6and 12 h after exposure to MeJA and immediately frozen in liquid nitrogen. Six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of hormone treatment Overall design: RNA from experimental sample and from the control sample (0 h) was used for hybridization to dual channel arrays. Two biological replicates were used for each experimental point. The quantification of each hybridization was submitted in two files, one for each slide side (technical replicates).

巴西CTC基因型SP80-3280的甘蔗单芽种茎，种植于盛有商用育苗基质（Plantmax，Eucatex）的200毫升塑料杯中，于温室条件下培养20天。随后将幼苗转移至生长箱中进行驯化，培养条件为26℃、16 h/8 h光暗周期，光子通量密度70 µE·m⁻²·s⁻¹。之后对幼苗喷施100 µmol·L⁻¹的茉莉酸甲酯（MeJA）溶液（购自美国康涅狄格州丹伯里市Bedoukian Research Inc.公司），对照组幼苗则喷施蒸馏水。分别于喷施MeJA后0、1、6、12小时采集叶片，并立即置于液氮中冷冻保存。每个时间点使用6株幼苗。针对每个样本混合组分别提取总RNA。关键词：激素处理时间进程。实验设计：以实验组样本及对照组（0小时）样本的RNA进行双通道基因芯片杂交。每个实验点设置2次生物学重复。每次芯片杂交的定量结果以两个文件提交，分别对应芯片的两个扫描面（技术重复）。