MOESM2 of ER stress regulates alkaline phosphatase gene expression in vascular smooth muscle cells via an ATF4-dependent mechanism

Additional file 2. Proteomics results. List of proteins, that bind the ALP promoter, identified by mass spectrometry. Nuclear extracts were bound and eluted off the biotinylated DNA on beads and analysed by LCâMS/MS. Raw mass spectrometry data were analysed in Proteome Discoverer (ThermoScientific; v1.3.0.339) utilising the Mascot database. Samples were searched against Uniprot database to identify proteins bound to the ALP promoter and compared with proteins that nonspecifically bound to unconjugated beads.

附加文件2：蛋白质组学结果。列出了通过质谱鉴定与ALP启动子结合的蛋白质列表。核提取物被结合并从磁珠上的生物素化DNA上洗脱下来，然后通过液相色谱-串联质谱（LC-MS/MS）进行分析。原始质谱数据在Proteome Discoverer软件（ThermoScientific；版本1.3.0.339）中进行分析，该软件利用Mascot数据库。样本与Uniprot数据库进行比对，以识别与ALP启动子结合的蛋白质，并与非特异性结合到未偶联磁珠的蛋白质进行比较。