Single-cell transcriptome profiling of Sox2-low iPS cells

Single-cell RNA-seq was performed on homozygous Sox2 knockout induced pluripotent stem (iPS) cells, where residual Sox2 expression is observed from incompletely silenced retroviral transgenes. These were compared to Sox2-low iPS cells rescued by exogenous Sox2 transgenic expression, and wild-type iPS cells to assess the lineage expression profile of Sox2-low cells and degree of transcriptional heterogeneity in each population.

本研究对纯合Sox2基因敲除诱导多能干细胞（iPS细胞）开展了单细胞RNA测序（Single-cell RNA-seq），此类细胞因逆转录病毒转基因未完全沉默，仍可检测到Sox2的残留表达。将该细胞系与经外源性Sox2转基因表达挽救的Sox2低表达iPS细胞，以及野生型iPS细胞进行对照，以分析Sox2低表达细胞的谱系表达谱，以及各细胞群体的转录异质性程度。