Next Generation Sequencing Facilitates Quantitative Analysis of SKOV3 cells overexpression of TNFAIP8L2

Purpose: Next generation sequencing (NGS) has revolutionized system-based analysis of cellular pathways. The goals of this study are to compare NGS-derived transcriptome profiling of human ovarian cancer cell, SKOV3 cells after endogenous overexpression of TNFAIP8L2 (TIPE2) . Methods: We constructed TIPE2 overexpression SKOV3 cell lines and control vector cell lines with lentivirus. Then the NGS based deep sequencing analysis was generated in triplicate to analyze the critical downstream targets after TIPE2 overexpression using ILLumina GAIIx. Results: The differentially expressed genes were identified using an optimized data analysis workflow, and we identified 49 upregulated and 28 downregulated genes in the SKOV3/TIPE2 group compared with the vector group using a |log2Foldchange|?0 and p < 0.05. Conclusions: Our study represents the first detailed analysis of transcriptomes in ovarian cancer cell line after overexpression of TIPE2 by RNA-seq technology. Overall design: mRNA profiles of SKOV3 cell overexpression of TIPE2

研究目的：下一代测序（Next Generation Sequencing, NGS）已彻底革新了细胞通路的系统性分析。本研究旨在对比内源性过表达TNFAIP8L2（TIPE2）后人卵巢癌细胞SKOV3的NGS转录组谱。
方法：本研究通过慢病毒构建TIPE2过表达SKOV3细胞系与空载体对照细胞系；随后采用Illumina GAIIx平台开展三次生物学重复的NGS深度测序分析，以探究TIPE2过表达后的关键下游靶基因。
结果：通过优化的数据分析流程鉴定差异表达基因，以|log₂(折叠变化)|≥0且p<0.05为筛选标准，相较于空载体对照组，SKOV3/TIPE2组中共鉴定出49个上调基因与28个下调基因。
结论：本研究首次通过RNA测序技术，对TIPE2过表达后的卵巢癌细胞系转录组进行了详尽分析。
整体实验设计：TIPE2过表达SKOV3细胞的mRNA表达谱。