Efferocytosis Fuels Requirements of Fatty Acid Oxidation and the Electron Transport Chain to Polarize Macrophages for Tissue Repair

Screening pathways that are affected in macrophages during efferocytosis primary peritoneal macrophages are cocultured with apoptotic cells or LPS for 5 hours; unengulfed apoptotic cells are rinsed off; macrophages are harvested and RNA was extracted for sequencing

为筛选胞葬作用（efferocytosis）过程中巨噬细胞内受影响的信号通路，将原代腹腔巨噬细胞与凋亡细胞或脂多糖（Lipopolysaccharide，LPS）共培养5小时；冲洗去除未被吞噬的凋亡细胞；收集巨噬细胞并提取RNA进行测序。