Differential Expression of Super-enhancer-associated Long Non-coding RNAs in Uterine Leiomyomas. Differential Expression of Super-enhancer-associated Long Non-coding RNAs in Uterine Leiomyomas

Arraystar SE-lncRNAs Arrays was used to systematically profile the differentially expressed SE-lncRNAs along with corresponding SE-regulated protein coding genes from eight leiomyomas and paired myometrium. The analysis indicated 7680 SE-lncRNAs were expressed, of which 721 SE-lncRNAs were increased, while 247 SE-lncRNAs were decreased by 1.5-fold or greater in leiomyoma. Thirteen novel SE-lncRNAs and their corresponding protein coding genes were selected, and their expression was confirmed in eighty-one paired leiomyoma tissues by quantitative real-time PCR. The thirteen pairs of SE-lncRNAs and their corresponding protein coding genes included RP11-353N14.2/CBX4, SOCS2-AS1/SOCS2, RP1-170O19.14/HOXA11, CASC15/PRL, EGFLAM-AS1/EGFLAM, RP11-225H22/NEURL1, RP5-1086K13.1/CD58, AC092839.3/SPTBN1, RP11-69I8.3/CTGF, TM4SF1-AS1/TM4SF1, RP11-373D23/FOSL2, RP11-399K21.11/COMTD1 and CTB-113P19.1/SPARC. Collectively, our results indicate that the differential expression of SE in fibroids is another mechanism contributing to dysregulation of protein coding genes in fibroids. Overall design: Identify the expression profile of SE-lncRNAs along with corresponding SE-regulated protein coding genes in leiomyomas and paired myometrium using Arraystar SE-lncRNAs Arrays

本研究采用Arraystar SE-lncRNAs芯片（Arraystar SE-lncRNAs Arrays），对8例子宫肌瘤组织及其配对子宫肌层组织中差异表达的超级增强子相关长链非编码RNA（SE-lncRNAs）及其相应的SE调控蛋白编码基因开展系统性表达谱分析。分析结果显示，共检测到7680个SE-lncRNAs的表达，其中721个SE-lncRNAs在子宫肌瘤组织中表达上调1.5倍及以上，另有247个SE-lncRNAs在肌瘤组织中表达下调1.5倍及以上。本研究筛选得到13个新发现的SE-lncRNAs及其对应的蛋白编码基因，并通过实时荧光定量PCR在81例配对子宫肌瘤组织中验证了这些基因的表达水平。这13对SE-lncRNAs及其对应的蛋白编码基因分别为：RP11-353N14.2/CBX4、SOCS2-AS1/SOCS2、RP1-170O19.14/HOXA11、CASC15/PRL、EGFLAM-AS1/EGFLAM、RP11-225H22/NEURL1、RP5-1086K13.1/CD58、AC092839.3/SPTBN1、RP11-69I8.3/CTGF、TM4SF1-AS1/TM4SF1、RP11-373D23/FOSL2、RP11-399K21.11/COMTD1以及CTB-113P19.1/SPARC。综上，本研究结果表明，肌瘤组织中超级增强子（SE）的差异表达是导致肌瘤中蛋白编码基因表达失调的又一重要机制。实验设计概述：采用Arraystar SE-lncRNAs芯片，对子宫肌瘤组织及其配对子宫肌层组织中的SE-lncRNAs及其相应的SE调控蛋白编码基因的表达谱进行鉴定。