Supplementary Tables 1 - 3 from Long-Range Chromatin Interactions Drive Mutant TERT Promoter Activation

Supplementary Table 1. Differential gene expression data list of BLM6 and BLM14 cells (-146C>T vs. -146C) for the genes that were predicted to be off-targets of the guide RNA that has been used for the CRISPR/Cas9 genome engineering. Supplementary Table 2. Table showing list of interacting regions obtained from 4C analysis. 4C was performed in BLM6 and BLM14 cells. Differential interactions were obtained from 4C analysis. List shows the genomic coordinates (column 1-3) and their Refseq identities (column 4) of regions interacting with mutant Tert promoter. Column 5 shows whether the regions were tested by 3C-qPCR assay. Y = Yes, N=No. Column 6 shows whether the region was removed by CRISPR/Cas9 editing in the later part of the study. * mark is named as T-INT1 region. Supplementary Table 3. Primer sequences for ChIP-qPCR, 4C and 3C and RT-qPCR experiments.

补充表1。本列表为BLM6与BLM14细胞中，以-146C>T与-146C为比较组的差异基因表达数据集，涉及本次CRISPR/Cas9基因组编辑所用向导RNA（guide RNA）的预测脱靶基因。
补充表2。本表格列出了4C染色体构象捕获（4C）分析得到的染色质互作区域列表。本次4C分析在BLM6与BLM14细胞中开展，通过该分析获得了差异互作区域。该列表包含与突变型Tert启动子互作区域的基因组坐标（第1至3列）及其RefSeq标识信息（第4列）；第5列标注了该区域是否经3C-qPCR实验验证，其中Y代表是，N代表否；第6列标注了该区域是否在本研究后续环节中通过CRISPR/Cas9编辑被移除。带*号的区域被命名为T-INT1区域。
补充表3。本表格列出了ChIP-qPCR、4C、3C以及RT-qPCR实验所用的引物序列。