The effect of Zinc defficiency on the mouse paneth cell transcriptome in TNF context

We investigated the effect of zinc depletion on the mouse intestine, and specifically on paneth cells. Mice were placed on a zinc depleted diet for 7 weeks after which they were given TNF for 3 hours and the the effects of dietary zinc shortage on paneth cells in TNF context were investigated. A zinc is an essential element for correct paneth cell function and zinc defficiency is a human and animal health problem investigating the PC transcriptom my provide valuable insights into the exact mechanism. Overall design: 2 goups with 4 biological replicates each were used: one group was given the standerd chow and one group was given zinc depleted food for 7 weeks, both were injected with TNF 3 hours before sampling

本研究探究了锌缺乏对小鼠肠道，尤其是潘氏细胞（Paneth cells）的影响。实验中，小鼠先经锌缺乏饲料喂养7周，随后予以肿瘤坏死因子（TNF）处理3小时，以此分析膳食锌缺乏在TNF处理背景下对潘氏细胞的作用。锌是维持潘氏细胞正常功能的必需元素，而锌缺乏是困扰人类与动物的健康问题；对潘氏细胞转录组（transcriptome）开展研究，可为解析其具体作用机制提供宝贵见解。实验设计概况：本实验设置两组，每组含4个生物学重复：一组给予标准饲料，另一组以锌缺乏饲料喂养7周，两组均在采样前3小时接受TNF注射。