'Nonpareil' almond genome and methylome sequencing. Prunus dulcis

Breeding and improvement efforts worldwide have led to the development of key, productive cultivars, including Nonpareil, which is the most commonly grown almond cultivar. Thus far, genomic resources for this species have been limited, and the whole genome assembly for Nonpareil is not currently available despite its economic importance and use in almond breeding. We generated a 615.89X coverage sequence using Illumina, PacBio, and optical mapping technologies. Gene prediction revealed 27,487 genes using MinION Oxford nanopore and Illumina RNA sequencing, and genome annotation found that 68% of predicted models are associated with at least one biological function. In addition to the genome sequence and annotation, this report also provides the complete methylome of several key almond tissues, including leaf, flower, endocarp, mesocarp, fruit skin, and seed coat.

全球范围内的育种与改良工作已培育出一批核心高产栽培品种，其中诺帕雷尔（Nonpareil）是目前种植最广泛的杏仁栽培品种。迄今为止，该物种的基因组资源仍较为有限；尽管诺帕雷尔具有重要经济价值且广泛应用于杏仁育种，但其全基因组组装目前仍未完成。本研究利用Illumina、PacBio及光学图谱技术，生成了覆盖度达615.89X的测序序列。依托MinION牛津纳米孔测序与Illumina RNA测序数据开展基因预测，共鉴定得到27487个基因；基因组注释结果显示，68%的预测基因模型至少对应一种生物学功能。除基因组序列与注释信息外，本报告还提供了杏仁多个关键组织的完整甲基化组数据，涵盖叶片、花、内果皮、中果皮、果皮及种皮。