Antioxidant Actions of Thymoquinone, Silymarin, and Curcumin on Experimental Aortic Ischemia-Reperfusion Model in Wistar Albino Rats

ABSTRACT Introduction: Medical improvements are needed to prevent ischemia-reperfusion injury in thoracoabdominal aortic surgery. The aim of this study was to determine the antioxidant effects of thymoquinone, silymarin, and curcumin against ischemia-reperfusion injury associated with abdominal aorta. Methods: Twenty-five Wistar albino rats were included in the study. Sham, control, and treatment (thymoquinone, silymarin, and curcumin) groups were set in equal numbers. Ischemia-reperfusion was applied by clamping (120 minutes) and de-clamping (60 minutes) the infrarenal aorta of all groups, except the sham group. Before reperfusion, thymoquinone, silymarin, and curcumin were given intraperitoneally to the treatment groups. After reperfusion, blood samples were taken from the right ventricle. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were studied in serum samples and histopathological examination was performed on the gastrocnemius muscle. Results: There was a significant difference in TOS and OSI values between the control and sham groups. Both values were found higher in the control group than in the sham group (P

摘要
引言：当前亟需研发医学干预手段以防治胸腹主动脉手术中的缺血再灌注损伤。本研究旨在探讨百里香醌（thymoquinone）、水飞蓟素（silymarin）与姜黄素（curcumin）对腹主动脉相关缺血再灌注损伤的抗氧化作用。
方法：本研究共纳入25只白化Wistar大鼠。设置假手术组、对照组与治疗组，治疗组分别给予百里香醌（thymoquinone）、水飞蓟素（silymarin）与姜黄素（curcumin），各组样本量均等。除假手术组外，其余各组均通过夹闭肾下腹主动脉120分钟、再灌注开放60分钟的方式构建缺血再灌注模型。再灌注开始前，治疗组大鼠经腹腔给予对应受试药物。再灌注结束后，经右心室采集血液样本。对血清样本检测总抗氧化能力（Total Antioxidant Status, TAS）、总氧化能力（Total Oxidant Status, TOS）及氧化应激指数（Oxidative Stress Index, OSI），并对腓肠肌组织开展组织病理学检查。
结果：对照组与假手术组的TOS与OSI值存在显著差异，对照组两项指标水平均高于假手术组（P