Impact of smoking on dendritic cells in patients with oral squamous cell carcinoma

Abstract Smoking has been shown to alter innate and adaptive immune responses and is directly associated with the onset of oral squamous cell carcinoma (OSCC). The purpose of this study was to evaluate the effect of cigarette smoke exposure on dendritic cells (DCs) from OSCC patients. CD1a and CD83 antibodies were used to identify immature and mature DCs, respectively, by immunohistochemistry in OSCC samples of 24 smokers and 24 non-smokers. Density of DCs was calculated in intra and peritumoral areas. Clinical and microscopic findings were reviewed and analyzed for all patients. Smokers with OSCC had a lower density of intra and peritumoral DCs when compared to non-smokers. Tumors classified as moderately/poorly differentiated had lower peritumoral CD1a+ DCs than well-differentiated tumors (p

摘要 吸烟已被证实可改变先天免疫与适应性免疫应答，且与口腔鳞状细胞癌（oral squamous cell carcinoma, OSCC）的发病直接相关。本研究旨在评估香烟烟雾暴露对口腔鳞状细胞癌患者树突状细胞（dendritic cells, DCs）的影响。研究采用免疫组化法，使用CD1a与CD83抗体分别识别24名吸烟者与24名非吸烟者的口腔鳞状细胞癌样本中的未成熟DC与成熟DC。分别计算瘤内及瘤周区域的树突状细胞密度。对所有患者的临床及镜下检查结果进行回顾与分析。与非吸烟者相比，罹患口腔鳞状细胞癌的吸烟者其瘤内及瘤周树突状细胞密度更低。中低分化肿瘤的瘤周CD1a+树突状细胞密度低于高分化肿瘤（p