RNA expression in KATOIII cells harboring biallelic deletions of the +22.6-kb site, epithelial-specific enchancer of ABO gene. RNA expression in KATOIII cells harboring biallelic deletions of the +22.6-kb site, epithelial-specific enchancer of ABO gene

Wild-type KATOIII cells and their derived mutant clones B3 and B4 harboring biallelic deletions of the +22.6-kb site, located 22.6 kb downstream from the ABO translation start site Overall design: CRISPR/Cas9 system with a pair of genome-editing plasmids 5′Enhancer and 3′Enhancer was used to create genomic deletions of the +22.6-kb site in KATOIII cells. The sequencing revealed genomic deletion of the sequences +22,579/+22,740 and +22,579/+22,742 in clone B3, and the sequences +22,580/+22,740 and +22,580/+22,742 in clone B4 from ABO translation start site.

野生型KATOIII细胞及其衍生的突变克隆B3、B4携带了位于ABO翻译起始位点下游22.6 kb处的+22.6-kb位点的双等位基因缺失。整体实验设计：采用CRISPR/Cas9系统，搭配一对基因组编辑质粒5′Enhancer与3′Enhancer，在KATOIII细胞中构建+22.6-kb位点的基因组缺失突变体。测序结果显示，相较于ABO翻译起始位点，克隆B3的基因组缺失序列为+22,579/+22,740与+22,579/+22,742，克隆B4的基因组缺失序列为+22,580/+22,740与+22,580/+22,742。