Mode of action analysis: Distinct Staphylococcus aureus transcriptome changes upon antivirulence lactone treatment

To elucidate the antivirulent lactone U1 mode of action, next generation sequencing was applied to analyze the transcriptome of NCTC 8325 cells treated with either compound or DMSO as control. Overall design: Two biological replicates for compound treated and also two biological replicates for DMSO treated Staphylococcus aureus cultures were analysed.

为阐明抗毒力内酯U1（antivirulent lactone U1）的作用模式，本研究采用下一代测序（next generation sequencing）技术，对经目标化合物或二甲基亚砜（DMSO）处理的金黄色葡萄球菌（Staphylococcus aureus）NCTC 8325菌株细胞进行转录组分析。
整体实验设计：针对经化合物处理的金黄色葡萄球菌培养物，以及经DMSO处理的对照组金黄色葡萄球菌培养物，分别设置2个生物学重复样本开展分析。