CIL:13117, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

High resolution micrograph of cubic membrane array which represents the cell's method of storing large quantities of lipid and protein bilayers into the smallest possible volume. These membranes can quickly expand into planar sheets when the ampulla or the collecting canal needs to expand. These arrays actually bury the microtubular ribbons between the membrane of the collecting canal and these three-dimensional arrays. TEM taken on 8/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Adapted with permission from the J. Cell Sci. 112:3733-3745, 1999. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

本图像为立方膜阵列的高分辨率显微照片，展示了细胞将大量脂质与蛋白质双层膜以最小体积储存的机制。当壶腹或收集小管需要扩张时，此类膜可快速延展为扁平片状结构。此类三维阵列实际上将微管带夹在收集小管膜与该阵列之间。本透射电子显微镜（Transmission Electron Microscope, TEM）图像拍摄于1996年8月8日，由R. Allen使用工作电压为80kV的蔡司（Zeiss）10A电镜拍摄，放大倍数为31500倍。经许可改编自1999年发表于《Journal of Cell Science》第112卷第3733-3745页的文章。原始胶片通过爱普生（Epson）Perfection V750 Pro扫描仪进行数字化扫描。本图像最适用于定量分析。样品制备采用标准流程：经戊二醛固定、四氧化锇后固定，以乙醇脱水后包埋于环氧树脂中，通过超薄切片机制备厚度约75nm的切片。更多相关信息可访问：http://www5.pbrc.hawaii.edu/allen/。