KU70 and KU80 genes in P. tetraurelia.

(A) Diagram of the WGD relationships between KU70 or KU80 genes in P. tetraurelia. The % of identity between genes (nt) and proteins (aa) are indicated along the arrows connecting two genes. (B) Transcription profiles of KU70 and KU80 genes during an autogamy time-course of strain 51, as determined by high-throughput RNA-seq. V: vegetative cells; S: starved or meiotic cells with intact parental MAC; T0: 50% of cells with fragmented MAC; the following time-points refer to hours after T0 [51]. On the vertical axis, FPKM represents the number of fragments per gene kb per million of fragments that were uniquely mapped on the genome. (C) Detection of KU70 and KU80c mRNA during autogamy, through northern blot hybridization. Control RNAi: RNAi against the nonessential ND7 gene [43], which encodes an exocytosis protein. V: vegetative cells. The times refer to hours after T0, the time at which 50% of cells have a fragmented MAC (Figure S1A).

(A) 四膜虫（*Paramecium tetraurelia*）中KU70或KU80基因间的全基因组复制（Whole Genome Duplication, WGD）关联示意图。连接两个基因的箭头旁标注了基因（核苷酸，nt）与蛋白（氨基酸，aa）间的序列一致性百分比。(B) 菌株51的自接合时间进程中KU70与KU80基因的转录谱，通过高通量RNA测序（RNA-sequencing, RNA-seq）测定。其中V代表营养期细胞；S代表饥饿细胞或亲本大核（Macronucleus, MAC）完整的减数分裂细胞；T0指50%细胞的大核发生片段化的时间点；后续时间点均以T0后的小时数标注[51]。纵轴的FPKM（Fragments Per Kilobase of transcript per Million mapped reads）为每百万唯一比对至基因组的片段中，每个基因每千碱基的片段数。(C) 自接合过程中KU70与KU80c mRNA的Northern印迹杂交检测。对照RNA干扰（RNA interference, RNAi）：靶向非必需基因ND7的RNA干扰[43]，ND7编码一种胞吐蛋白。V代表营养期细胞，时间点均以T0后的小时数标注——T0即50%细胞的大核发生片段化的时间点（图S1A）。