Genome wide analysis of genes expressed in late G1/S phase of the cell cycle in PMCA4 knockout (P4KO) and P4KO rescued with PMCA4a primary vascular smooth muscle cells

Husain lab cell proliferation in vitro experimental data showed that electroporating PMCA4a cDNA into P4KO cells rescued the G1 arrest phenotype in P4KO primary vascular smooth muscle cells (VSMC). This microarray documents the different gene expression profiles of P4KO and P4KO+PMCA4a VSMC at the late G1/S stage in serum synchronized cell populations (n=4 populations for each genotype). Serum synchronized late G1/S stage cell populations (P4KO and P4KO+PMCA4a) were employed to extract total RNA which was used for microarray analysis.

Husain实验室的体外细胞增殖实验数据表明，将PMCA4a cDNA电转染至P4KO细胞后，可挽救P4KO原代血管平滑肌细胞（vascular smooth muscle cells, VSMC）的G1期阻滞表型。本基因微阵列数据集记录了血清同步化细胞群中处于G1/S晚期的P4KO与P4KO+PMCA4a组VSMC的差异基因表达谱（每种基因型对应4个细胞群）。研究人员通过上述血清同步化的G1/S晚期细胞群（P4KO组与P4KO+PMCA4a组）提取总RNA，用于后续的基因微阵列分析。